Anti-Elastase, Neutrophil, Antibody

Mouse Monoclonal Antibody specific to Elastase, Neutrophil

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 3.4.21.37, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, HLE, Medullasin, PMN elastase

Gene Name

ELANE

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=ELANE">ELANE</a>

UniProt

P08246

Cellular Locus

Cytoplasmic vesicle, phagosome

Host

Mouse

Reactivity

Human

Immunogen

Purified human neutrophil elastase.

Target Antigen

Neutrophil elastase

Target

Elastase, Neutrophil,

Clonality

Monoclonal

Isotype

IgG2b

Type

Antibody

Applications

ELISA, Neutralization

Field of Research

Enzymes

Purification Method

Purified by protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984). {PubMed:10947984, PubMed:15140022}.

Additionnal Information

These antibodies have been qualified for use in ELISA to detect human neutrophil elastase and in studies of inhibition and enzyme kinetics of human neutrophil elastase.