Anti-VGlut2 Transporter Antibody

Mouse Monoclonal Antibody specific to VGlut2 Transporter

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

VGluT2, Differentiation-associated BNPI, Differentiation-associated Na(+-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 6

Gene Name

Slc17a6

NCBI Gene ID

UniProt

Q9JI12

Cellular Locus

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Cell junction, synapse, synaptosome

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 501-582 (cytoplasmic C-terminus) of rat VGlut2 (accession no. NP_445879).

Target Antigen

Vesicular glutamate transporter 2

Target

VGlut2 Transporter

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells (PubMed:11551935, PubMed:11698619, PubMed:11698620, PubMed:11502256, PubMed:17046815, PubMed:18080752). May also mediate the transport of inorganic phosphate (PubMed:17046815). Involved in the regulation of retinal hyaloid vessel regression during postnatal development (By similarity). May also play a role in the endocrine glutamatergic system of other tissues such as pineal gland and pancreas (PubMed:11551935). {UniProtKB:Q8BLE7, PubMed:11502256, PubMed:11551935, PubMed:11698619, PubMed:11698620, PubMed:17046815, PubMed:18080752}.

Additionnal Information

Immunoblotting: use at 1-2ug/mL. A band of ~60kDa is detected. Immunohistochemistry: use at 1-5ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: rat brain lysate.

Storage Conditions

This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat VGlut2.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

The ATP-dependent, chloride-sensitive vesicular glutamate transporters include VGlut1, VGlut2, and VGlut3. VGlut1 and VGlut2 are expressed in brain in a complementary fashion. VGlut1 is expressed in the telencephalic regions whereas VGlut2 is expressed in the lower brainstem and diencephalic regions. VGlut3 is expressed in a small number of neurons in the striatum, hippocampus, cerebral cortex, and raphe nuclei. Pancreatic alpha and beta cells express VGlut1 and VGlut2 in response to glucose concentrations.