UltraFast Biotinylation Kit (microgram scale, 5 reactions)

<strong>UltraFast Antibody and Protein Biotinylation Kit (microgram scale, 5 reactions)</strong>_x000D_ <strong>Catalog number:</strong> K2010011M_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Size:</strong> 5 reactions_x000D_ <strong>Supplied as:</strong> Ready-to-use kit_x000D_ <strong>Storage:</strong> 2-8°C_x000D_ _x000D_ <strong>Applications:</strong> for custom protein and antibody biotinylation in vitro. 45 min ultrafastand highly efficient protocol. This kit is for the biotinylation of microgram amounts (10-1000 ug). The kit contains all the required materials for 5 independent biotinylation reactions. Components include NHS-activated Biotin Linkers, biotinylation and storage buffers as well as  size-exlcusion columns for the removal of free unconjugated biotin. Kit has been validated in applications that include ELISA, CLIA, enzymatic tagging and more._x000D_ _x000D_ <strong>Keywords:</strong> Biotinylation Kits, Biotin labelling kit, biotin tagging kit, biotin conjugation kit_x000D_ <strong>Grade:</strong> Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered on a 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: De Munter S, Görnemann J, Derua R, Lesage B, Qian J, Heroes E, Waelkens E,_x000D_ Van Eynde A, Beullens M, Bollen M. Split-BioID: a proximity biotinylation assay_x000D_ for dimerization-dependent protein interactions. FEBS Lett. 2017_x000D_ Jan;591(2):415-424._x000D_ _x000D_ 2: Hung V, Lam SS, Udeshi ND, Svinkina T, Guzman G, Mootha VK, Carr SA, Ting AY._x000D_ Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in_x000D_ living human cells by proximity biotinylation. Elife. 2017 Apr 25;6:e24463._x000D_ _x000D_ 3: Kaewsapsak P, Shechner DM, Mallard W, Rinn JL, Ting AY. Live-cell mapping of_x000D_ organelle-associated RNAs via proximity biotinylation combined with protein-RNA_x000D_ crosslinking. Elife. 2017 Dec 14;6:e29224._x000D_ _x000D_ 4: Beckett D, Kovaleva E, Schatz PJ. A minimal peptide substrate in biotin_x000D_ holoenzyme synthetase-catalyzed biotinylation. Protein Sci. 1999 Apr;8(4):921-9._x000D_ _x000D_ 5: Rice GM, Nutiu R, Gampe CM. Direct Chemical Biotinylation of RNA 5'-Ends_x000D_ Using a Diazo Reagent. Methods Mol Biol. 2019;1870:81-87._x000D_ _x000D_ 6: Fouda AE, Embogama DM, Ramanayake-Mudiyanselage V, H Pflum MK. Chitosan-_x000D_ assisted permeabilization of ATP-biotin for live cell kinase-catalyzed_x000D_ biotinylation. Biotechniques. 2018 Sep;65(3):143-148._x000D_ _x000D_ 7: Trinh LA, Chong-Morrison V, Sauka-Spengler T. Biotagging, an in vivo_x000D_ biotinylation approach for cell-type specific subcellular profiling in_x000D_ zebrafish. Methods. 2018 Nov 1;150:24-31._x000D_ _x000D_ 8: Varnaitė R, MacNeill SA. Meet the neighbors: Mapping local protein_x000D_ interactomes by proximity-dependent labeling with BioID. <a href="https://pubmed.ncbi.nlm.nih.gov/27329485/">Proteomics</a>. 2016_x000D_ Oct;16(19):2503-2518._x000D_ _x000D_ <strong>Products Related to UltraFast Antibody and Protein Biotinylation Kit (microgram scale, 5 reactions)</strong>: <a href="https://moleculardepot.com/product-category/research-kits/">Research Kits</a>