Indole-3-Acetic Acid (IAA) Solution, 1mg/mL, Sterile

This substance plays crucial roles in various aspects of plant physiology, including cell enlargement, division, tissue differentiation, and responses to environmental stimuli such as light and gravity. In plant tissue culture, it fosters the development of organs such as shoots and roots. In horticulture, it is used to promote adventitious root growth. It functions as a vital supplement in growth media formulations like MS media or Gamborg’s media, facilitating callus initiation. Auxin sprays containing IAA can be used in some crops to improve fruit sets and prevent premature fruit drop.

Follow the given steps to prepare the tissue culture media using PCT’s IAA solution: Prepare liquid tissue culture media using PCT’s MS, DKW, or any other alternative media. Add sugar based on the quantity of media you’re making (generally for 1-liter media 30 grams of sugar is added), 1 ml/L PPM, and 3-4 grams per liter of gellan gum or 6-8 grams per liter of agar. Then, add the desired quantity of the plant hormone. Autoclave the medium for 20 minutes at 15 psi and 121°C.

Easy to use Used as a supplement in growth media like MS media or Gamborg’s media for callus initiation. Involved in both primary and lateral root initiation. Regulate growth and developmental processes such as cell division, elongation, and tissue differentiation.