Recombinant Avian infectious bronchitis virus Non-structural protein 5b (5b)
Product Specifications
Product Name Alternative
(ns5b) (Accessory protein 5b)
Abbreviation
Recombinant Avian infectious bronchitis virus Non-structural protein 5b
Gene Name
5b
UniProt
Q80RZ3
Expression Region
1-82aa
Organism
Avian infectious bronchitis virus (strain M41) (IBV)
Target Sequence
MNNSKDNPFCGAIARKARIYLREGLDCVYFLNKAGQAESCPACTSLVFQGKTCEEHKYNNNLLSWQAVRQLERQMPQLQSSN
Tag
C-terminal 6xHis-tagged
Type
Developed Protein
Source
E.coli
Field of Research
Others
Relevance
Involved in host translation shutoff without degradating host RNA. By suppressing host gene expression, facilitates the evasion from host type I interferon immune response.
Endotoxin
Not test
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
11.8 kDa
References & Citations
"Infectious Bronchitis Coronavirus Limits Interferon Production by Inducing a Host Shutoff That Requires Accessory Protein 5b." Kint J., Langereis M.A., Maier H.J., Britton P., van Kuppeveld F.J., Koumans J., Wiegertjes G.F., Forlenza M. J. Virol. 90:7519-7528 (2016)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Product MSDS
https://www.cusabio.com/msds/12932610/
Protein Length
Full Length
Available Sizes
Curated Selection
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