Recombinant Avian infectious bronchitis virus Non-structural protein 5b (5b)

Product Specifications

Product Name Alternative

(ns5b) (Accessory protein 5b)

Abbreviation

Recombinant Avian infectious bronchitis virus Non-structural protein 5b

Gene Name

UniProt

Q80RZ3

Expression Region

1-82aa

Organism

Avian infectious bronchitis virus (strain M41) (IBV)

Target Sequence

MNNSKDNPFCGAIARKARIYLREGLDCVYFLNKAGQAESCPACTSLVFQGKTCEEHKYNNNLLSWQAVRQLERQMPQLQSSN

Tag

C-terminal 6xHis-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

Involved in host translation shutoff without degradating host RNA. By suppressing host gene expression, facilitates the evasion from host type I interferon immune response.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

11.8 kDa

References & Citations

"Infectious Bronchitis Coronavirus Limits Interferon Production by Inducing a Host Shutoff That Requires Accessory Protein 5b." Kint J., Langereis M.A., Maier H.J., Britton P., van Kuppeveld F.J., Koumans J., Wiegertjes G.F., Forlenza M. J. Virol. 90:7519-7528 (2016)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.