Human Apolipoprotein H Protein, His Tag (MALS verified)

Autoantibodies directed to negatively charged phospholipids, in particular cardiolipin, have been detected in the serum of patients with systemic lupus erythematosus (SLE) and antiphospholipid syndrome (APS) . APS is characterised by venous and arterial thrombosis, recurrent spontaneous abortions and thrombocytopenia. It is now known that a serum cofactor, β2-glycoprotein 1, is required for the binding of cardiolipin by autoantibodies in the sera of patients with APS. By contrast, anticardiolipin antibodies from patients with infectious diseases (in particular, syphilis) do not require this cofactor. β2-Glycoprotein 1, also known as apolipoprotein H, is a relatively abundant serum protein (present at a concentration of about 0.2 mg/ml) that may play a role in coagulation. It has been shown to bind to platelets, mitochondria and negatively charged substances such as heparin, DNA, dextran sulphate and negatively charged phospholipids.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 38.1 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P02749

Expression Region

Gly 20 - Cys 345

Host

HEK293

Target

Apolipoprotein H

Conjugation

Unconjugated

Tag

C-10xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human Apolipoprotein H Protein, His Tag (Cat. No. APH-H52H3) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LDL R, His,Avitag (Cat. No. LDR-H82E7) with a linear range of 0.005-0.156 μg/mL (QC tested).