Sirtuin 1 (SIRT-1) Activity Assay Kit
Product Specifications
Type
Fluorometric method
Field of Research
Amino Acids and Proteins
Assay Principle
This kit uses the fluorescence resonance energy transfer (FRET) method for detection. After deacetylation by SIRT-1, the fluorescent substrate generates an activated fluorescent substrate, which can be decomposed by proteases to produce fluorescent substances. The maximum excitation wavelength of fluorescent substances is 340 nm, and the maximum emission wavelength is 440 nm.
Assay Performance Time
70 min
Sample Type
Animal tissue; cell
Detection Range
0.03-9.66 U/L
Sensitivity
0.03 U/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
8.1-9.9%
Intra CV
3.0-5.0%
Detection Instrument
Fluorescence Microplate reader
Available Sizes
Documents
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