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Sirtuin 1 (SIRT-1) Activity Assay Kit

Product Specifications

Type

Fluorometric method

Field of Research

Amino Acids and Proteins

Assay Principle

This kit uses the fluorescence resonance energy transfer (FRET) method for detection. After deacetylation by SIRT-1, the fluorescent substrate generates an activated fluorescent substrate, which can be decomposed by proteases to produce fluorescent substances. The maximum excitation wavelength of fluorescent substances is 340 nm, and the maximum emission wavelength is 440 nm.

Assay Performance Time

70 min

Sample Type

Animal tissue; cell

Detection Range

0.03-9.66 U/L

Sensitivity

0.03 U/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

8.1-9.9%

Intra CV

3.0-5.0%

Detection Instrument

Fluorescence Microplate reader

Available Sizes

Frequently Asked Questions

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