CHAPS Detergent (Biochemistry Grade)

<strong>CHAPS</strong>_x000D_ <strong>Catalog number:</strong> B2010157_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Volume/Weight:</strong> 1 g_x000D_ <strong>pH:</strong> na_x000D_ <strong>Supplied as:</strong> Ready-to-use_x000D_ <strong>Appearance:</strong> Solution_x000D_ <strong>Applications:</strong> multipurpose non-denaturing zwitterionic detergent. CHAPS has many applications in protein solubilization (especially membrane proteins). It has been used in molecular biology, biochemistry and clinical chemistry._x000D_ <strong>Storage:</strong> Room temperature_x000D_ <strong>Keywords:</strong> 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS_x000D_ <strong>Grade:</strong> Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered on a 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Tao H, Liu W, Simmons BN, Harris HK, Cox TC, Massiah MA. Purifying natively_x000D_ folded proteins from inclusion bodies using sarkosyl, Triton X-100, and CHAPS._x000D_ Biotechniques. 2010 Jan;48(1):61-4._x000D_ _x000D_ 2: Kieber M, Ono T, Oliver RC, Nyenhuis SB, Tieleman DP, Columbus L. The_x000D_ Fluidity of Phosphocholine and Maltoside Micelles and the Effect of CHAPS._x000D_ Biophys J. 2019 May 7;116(9):1682-1691._x000D_ _x000D_ 3: Massiah MA, Wright KM, Du H. Obtaining Soluble Folded Proteins from Inclusion_x000D_ Bodies Using Sarkosyl, Triton X-100, and CHAPS: Application to LB and M9 Minimal_x000D_ Media. Curr Protoc Protein Sci. 2016 Apr 1;84:6.13.1-6.13.24._x000D_ _x000D_ 4: Hwang E, Cheong HK, Kim SY, Kwon O, Blain KY, Choe S, Yeo KJ, Jung YW, Jeon_x000D_ YH, Cheong C. Crystal structure of the EnvZ periplasmic domain with CHAPS. FEBS_x000D_ Lett. 2017 May;591(10):1419-1428._x000D_ _x000D_ 5: Rodi PM, Bocco Gianello MD, Corregido MC, Gennaro AM. Comparative study of_x000D_ the interaction of CHAPS and Triton X-100 with the erythrocyte membrane. Biochim_x000D_ Biophys Acta. 2014 Mar;1838(3):859-66._x000D_ _x000D_ 6: Naranjo AN, McNeely PM, Katsaras J, Robinson AS. Impact of purification_x000D_ conditions and history on A2A adenosine receptor activity: The role of CHAPS and_x000D_ lipids. Protein Expr Purif. 2016 Aug;124:62-7._x000D_ _x000D_ 7: Herrera FE, Garay AS, Rodrigues DE. Structural properties of CHAPS micelles,_x000D_ studied by molecular dynamics simulations. J Phys Chem B. 2014 Apr_x000D_ 10;118(14):3912-21._x000D_ _x000D_ 8: Qin X, Liu M, Yang D, Zhang X. Concentration-dependent aggregation of CHAP S_x000D_ investigated by NMR spectroscopy. J Phys Chem B. 2010 Mar 25;114(11):3863-8._x000D_ _x000D_ 9: Musante L, Saraswat M, Duriez E, Byrne B, Ravidà A, Domon B, Holthofer H._x000D_ Biochemical and physical characterisation of urinary nanovesicles following_x000D_ CHAPS treatment. PLoS One. 2012;7(7):e37279._x000D_ _x000D_ 10: Qin X, Liu M, Zhang X, Yang D. Proton NMR based investigation of the effects_x000D_ of temperature and NaCl on micellar properties of CHAPS. J Phys Chem B. 2011 Mar_x000D_ 10;115(9):1991-8._x000D_ _x000D_ 11: Yao S, Westphal D, Babon JJ, Thompson GV, Robin AY, Adams JM, Colman PM,_x000D_ Czabotar PE. NMR studies of interactions between Bax and BH3 domain-containing_x000D_ peptides in the absence and presence of CHAP S. Arch Biochem Biophys. 2014 Mar_x000D_ 1;545:33-43._x000D_ _x000D_ 12: Naskar B, Ghosh S, Nagadome S, Sugihara G, Moulik SP. Behavior of the_x000D_ amphiphile CHAP S alone and in combination with the biopolymer inulin in water_x000D_ and isopropanol-water media. <a href="https://pubmed.ncbi.nlm.nih.gov/21667919/">Langmuir</a>. 2011 Aug 2;27(15):9148-59._x000D_ _x000D_ <strong>Products Related to CHAPS </strong> <a>: Biochemical Buffers and Solutions</a>