Coronavirus Enhanced Rapid RT-qPCR Detection Kit

BioVision’s Coronavirus Enhanced RT-qPCR Detection Kit is a real-time reverse transcription-polymerase chain reaction (RT-qPCR) test used for the qualitative detection of RNA from SARS-CoV-2 in human nasopharyngeal and oropharyngeal swab specimens. It allows the efficient cDNA synthesis and qPCR in a single tube. This kit includes a 2X RT-qPCR Mastermix that contains all the reagents supplied in a 2X concentration to perform the qPCR. A separate RT-qPCR Enzyme Mix for cDNA synthesis is included the kit. Additionally, the kit contains a vial of primers and probes. The detection Kit uses real time PCR fluorescence technology to specifically detect S and N genes from SARS-CoV-2. During the amplification process, the probes included will anneal to the specific target sequence located between the forward and reverse primers. The probe is then cleaved, releasing the reporter dye and generating a fluorescent signal. An internal control primer and probe set (Actin) is included to monitor proper specimen collection and assay setup.