Mouse anti Human CD23, conjugated with FITC

CD23 antigen is an integral membrane glycoprotein, Mr. 45 kdaltons The CD23 antigen is present on approximately all pre-B lymphocytes, plasma cells CD25 positive Ki-1 cells and HLA-DR. CD23 antigen is also expressed on Lymphoma, T Cell Lymphomas and B-CLLs. CD23 antigen is present on HodgkinÕs and Reed Sternberg cells).

PBMC: Add10 µL of MAB/106 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2o to 8oC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature (20oC). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

1. CD23: a multi-lineage cell activation molecule with a split personality. Int J Clin Lab Res 1992; 22(2):73-80Malavasi F; Funaro A; Alessio M; DeMonte LB; Ausiello CM; Dianzani U; Lanza F; Magrini E; Momo M; Roggero S 2. Eleven lymphoid phenotypic markers in HIV infection: selective changes induced by zidovudine treatment. J Acquir Immune Defic Syndr 1992;5(9):890-7Bass HZ; Hardy WD; Mitsuyasu RT; Wang YX; Cumberland W; Fahey JL 3. Three-color cytofluorometric analysis of CD8 cell subsets in HIV-1 infection. J Acquir Immune Defic Syndr 1991;4(12):1227-32 Prince HE; Jensen ER