Human Carbonic Anhydrase IX / CA9 (138-414) Protein, His Tag (MALS verified)

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. There are at least five distinct CA families (α, β, γ, δ and ε) . Carbonic anhydrase 9 (CA9 / CAIX) is also known as Membrane antigen MN (MN), Renal cell carcinoma-associated antigen G250, which belongs to the alpha-carbonic anhydrase family. CA9 / CAIX with an optimal activity at pH 6.49. Reversible hydration of carbon dioxide. CA IX participates in pH regulation. CA9 may be involved in the control of cell proliferation and transformation. CA-IX appears to be a novel specific biomarker for a cervical neoplasia.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 30.9 kDa. The protein migrates as 34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

NP_001207.2

Expression Region

Gln 138 - Asp 414

Host

HEK293

Target

Carbonic Anhydrase IX

Conjugation

Unconjugated

Tag

C-6xHis

Source

Human

Stability

The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human Carbonic Anhydrase IX (138-414), His Tag (Cat. No. CA9-H5220) at 1 μg/mL (100 μL/well) can bind Anti-Carbonic Anhydrase IX Antibody, Human IgG1 (Cat. No. AM213) with a linear range of 0.3-1.2 ng/mL (QC tested).