Chicken anti Staphylococcal Protein A, conjugated with HRP
Product Specifications
CAS Number
9007-83-4
Host
Chicken
Species Reactivity
Bacteria
Conjugation
HRP Conjugate
Type
Primary Antibodies
Source
Chickens were immunized with purified recombinant protein A. After multiple immunizations in Freunds adjuvant, eggs were collected and the IgY fraction purified. Antibodies were immunoaffinity-purified from a protein A agarose column and conjugated to horseradish peroxidase (HRP).
Applications
WB, ELISA
Field of Research
Toxic Compounds, Infectious Agents and Disease, Microbiology
Purification Method
Egg-yolk derived IgY Affinity Purified
Assay Principle
This antibody is suitable for ELISA and immuoblotting. Recommended starting dilutions for ELISA capture is 1:100 - 1:500 and for blotting, 1:5,000 - 1:20,000.
Concentration
See vial for Concentration
Form
Phosphate Buffered Saline‚ 50% Glycerol with 0,1% 2-Chloroacetamide
Precautions
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
References & Citations
1. Freiherr von Roman M1, Koller A, von Rüden D, Berensmeier S. 2014. Improved extracellular expression and purification of recombinant Staphylococcus aureus protein A. Protein Expr Purif. 93:87-92
Shipping Conditions
Ambient Temperature
Storage Conditions
Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
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