Human LGR5 / GPR49 protein, His Tag
LGR5 (also known as GPR49) is a seven-transmembrane protein of the class A Rhodopsin-like family of GPCRs. LGR5 and LGR4 bind the R-spondins with high affinity and mediate the potentiation of Wnt/β-catenin signaling by enhancing Wnt-induced LRP6 phosphorylation. The LGR5/RSPO complex could promote Wnt signalling via the neutralization of two transmembrane E3 ligases, RNF43 and ZNRF3.
Product Specifications
Background
LGR5 (also known as GPR49) is a seven-transmembrane protein of the class A Rhodopsin-like family of GPCRs. LGR5 and LGR4 bind the R-spondins with high affinity and mediate the potentiation of Wnt/β-catenin signaling by enhancing Wnt-induced LRP6 phosphorylation. The LGR5/RSPO complex could promote Wnt signalling via the neutralization of two transmembrane E3 ligases, RNF43 and ZNRF3.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 62.0 kDa. The protein migrates as 65-75 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
O75473-1
Expression Region
Gly 22 - Arg 561
Host
HEK293
Target
LGR5
Conjugation
Unconjugated
Tag
C-10xHis
Source
Human
Stability
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
90%
Bioactivity
Immobilized Human R-Spondin 3 Protein, Fc Tag (Cat. No. RS3-H5253) at 5 μg/mL (100 μL/well) can bind Human LGR5, His Tag (Cat. No. LG5-H52H3) with a linear range of 10-78 ng/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
62.0 kDa
Additionnal Information
Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
250ug*4
Host or Source
HEK293
Species
Human
Protein ID
O75473-1
Preservative
Trehalose
More Discoveries
Explore Other Products
Browse additional items from our catalog
123636-ML650 100 µL
Aspartate Aminotransferase (Cytoplasmic, Transaminase A, Glutamate Oxaloacetate Transaminase 1, GOT1) (MaxLight 650)
Sign In for Pricing
View Details 
HP208252 200 Reactions
Cyclophilin 40 (PPID) Human qPCR Primer Pair (NM_005038)
Sign In for Pricing
View Details APRab03833-01 20 µL
Phospho-IKB alpha (Ser32/Ser36) Rabbit Polyclonal Antibody
Sign In for Pricing
View Details 
APRab03833-02 50 µL
Phospho-IKB alpha (Ser32/Ser36) Rabbit Polyclonal Antibody
Sign In for Pricing
View Details APRab03833-03 100 µL
Phospho-IKB alpha (Ser32/Ser36) Rabbit Polyclonal Antibody
Sign In for Pricing
View Details