Human LGR5 / GPR49 protein, His Tag
LGR5 (also known as GPR49) is a seven-transmembrane protein of the class A Rhodopsin-like family of GPCRs. LGR5 and LGR4 bind the R-spondins with high affinity and mediate the potentiation of Wnt/β-catenin signaling by enhancing Wnt-induced LRP6 phosphorylation. The LGR5/RSPO complex could promote Wnt signalling via the neutralization of two transmembrane E3 ligases, RNF43 and ZNRF3.
Product Specifications
Background
LGR5 (also known as GPR49) is a seven-transmembrane protein of the class A Rhodopsin-like family of GPCRs. LGR5 and LGR4 bind the R-spondins with high affinity and mediate the potentiation of Wnt/β-catenin signaling by enhancing Wnt-induced LRP6 phosphorylation. The LGR5/RSPO complex could promote Wnt signalling via the neutralization of two transmembrane E3 ligases, RNF43 and ZNRF3.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 62.0 kDa. The protein migrates as 65-75 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
O75473-1
Expression Region
Gly 22 - Arg 561
Host
HEK293
Target
LGR5
Conjugation
Unconjugated
Tag
C-10xHis
Source
Human
Stability
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
90%
Bioactivity
Immobilized Human R-Spondin 3 Protein, Fc Tag (Cat. No. RS3-H5253) at 5 μg/mL (100 μL/well) can bind Human LGR5, His Tag (Cat. No. LG5-H52H3) with a linear range of 10-78 ng/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
62.0 kDa
Additionnal Information
Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
250ug*4
Host or Source
HEK293
Species
Human
Protein ID
O75473-1
Preservative
Trehalose
More Discoveries
Explore Other Products
Browse additional items from our catalog
43895094 4x 500 ng
Slc16a13-set siRNA/shRNA/RNAi Lentivector (Mouse)
Sign In for Pricing
View Details bsm-62057R-BF405-01 20 µL
CEND1 Recombinant Antibody, AbBy Fluor™ 405 Conjugated
Sign In for Pricing
View Details 
bsm-62057R-BF405-02 100 µL
CEND1 Recombinant Antibody, AbBy Fluor™ 405 Conjugated
Sign In for Pricing
View Details NBP2-54436-100ug 100 µg
Mouse Monoclonal Clathrin light chain Antibody (CLC/1421) - Azide and BSA Free
Sign In for Pricing
View Details