Malachite Green ELISA Kit

Malachite Green is a triarylmethane dye that applications in numerous industries. It is used in the pigment industry wherein it is used as a dye to color silk, paper, wool, and leather. In the aquaculture industry, it is used as an ectoparasiticide, fungicide, and antiseptic. It is also widely used as a food coloring agent and food additive. However, the use of malachite green has been controversial due to its toxic effects. The dye is reported to cause mutagenesis, carcinogenesis, immunological, reproductive, and respiratory toxicity. BioVision’s Malachite Green ELISA kit is used to quantitatively measure Malachite Green in muscle tissue samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Malachite Green antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Malachite Green in the samples.