Cas9 Protein

<strong>Cas9 Protein</strong>_x000D_ <strong>Catalog number:</strong> B2016726_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 50 µg_x000D_ <strong>Molecular Weight or Concentration:</strong> 161.2 kDa_x000D_ <strong>Supplied as:</strong> Solution_x000D_ <strong>Applications:</strong> a molecular tool for various biochemical applications_x000D_ <strong>Storage:</strong> -80°C_x000D_ <strong>Keywords:</strong> Recombinant Cas9 Protein_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Carmignotto GP, Azzoni AR. On the expression of recombinant Cas9 protein in E. coli BL21(DE3) and BL21(DE3) Rosetta strains J Biotechnol. 2019 Dec 20;306:62-70._x000D_ 2: Singpant P, Tubsuwan A, Sakdee S, Ketterman AJ, Jearawiriyapaisarn N, Kurita R, Nakamura Y, Songdej D, Tangprasittipap A, Bhukhai K, Chiangjong W, Hongeng S, Saisawang C. Recombinant Cas9 protein production in an endotoxin-free system and evaluation with editing the BCL11A gene in human cells Protein Expr Purif. 2023 Oct;210:106313._x000D_ 3: Yu X, Liang X, Xie H, Kumar S, Ravinder N, Potter J, de Mollerat du Jeu X, Chesnut JD. Improved delivery of Cas9 protein/gRNA complexes using lipofectamine CRISPRMAX Biotechnol Lett. 2016 Jun;38(6):919-29._x000D_ 4: Xue C, Greene EC. DNA Repair Pathway Choices in CRISPR-Cas9-Mediated Genome Editing Trends Genet. 2021 Jul;37(7):639-656._x000D_ 5: Liang X, Potter J, Kumar S, Zou Y, Quintanilla R, Sridharan M, Carte J, Chen W, Roark N, Ranganathan S, Ravinder N, Chesnut JD. Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection J Biotechnol. 2015 Aug 20;208:44-53._x000D_ 6: Albadri S, Del Bene F, Revenu C. Genome editing using CRISPR/Cas9-based knock-in approaches in zebrafish Methods. 2017 May 15;121-122:77-85._x000D_ 7: Seki A, Rutz S. Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells J Exp Med. 2018 Mar 5;215(3):985-997._x000D_ 8: Truong DJ, Kühner K, Kühn R, Werfel S, Engelhardt S, Wurst W, Ortiz O. Development of an intein-mediated split-Cas9 system for gene therapy Nucleic Acids Res. 2015 Jul 27;43(13):6450-8._x000D_ 9: Laughery MF, Wyrick JJ. Simple CRISPR-Cas9 Genome Editing in Saccharomyces cerevisiae Curr Protoc Mol Biol. 2019 Dec;129(1):e110._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/29892067">10: Haapaniemi E, Botla S, Persson J, Schmierer B, Taipale J. CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response Nat Med. 2018 Jul;24(7):927-930. </a>_x000D_ _x000D_ <strong>Products Related to Cas9 Protein can be found at</strong> <a href="https://moleculardepot.com/product-category/Proteins/"> Proteins</a>