Thiazolyl Blue

Thiazolyl Blue (MTT) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis[1].

Product Specifications

CAS Number

[298-93-1]

Product Name Alternative

MTT; Thiazolyl Blue Tetrazolium (bromide) ; Methylthiazolyldiphenyl-tetrazolium (bromide)

UNSPSC

12171500

Hazard Statement

H315, H319, H335, H341

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/MTT.html

Purity

99.84

Solubility

DMSO : 25 mg/mL (ultrasonic; warming; heat to 60°C) |H2O : 0.88 mg/mL (ultrasonic; warming)

Smiles

CC1=C(C)N=C(N2N=C(C3=CC=CC=C3)N=[N+]2C4=CC=CC=C4)S1.[Br-]

Molecular Formula

C18H16BrN5S

Molecular Weight

414.32

Precautions

H315, H319, H335, H341

References & Citations

[1]Berridge MV, et al. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 2005;11:127-52.|[2]Berridge MV, et al. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 2005;11:127-52.