Anti-Rhodopsin Antibody

Mouse Monoclonal Antibody specific to Rhodopsin

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Rhodopsin Mouse Monoclonal Antibody

Gene Name

RHO

NCBI Gene ID

UniProt

P02699

Cellular Locus

Membrane, Cell projection, cilium, photoreceptor outer segment

Host

Mouse

Reactivity

All Mammalian Species Tested, Most Fish, Birds, Amphibians

Immunogen

Bovine rhodopsin

Target Antigen

Rhodopsin

Target

Rhodopsin

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF, IP, ELISA

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth (By similarity). Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins (PubMed:10926528, PubMed:12044163, PubMed:11972040, PubMed:16908857, PubMed:16586416, PubMed:17060607, PubMed:17449675, PubMed:18818650, PubMed:21389983, PubMed:22198838, PubMed:23579341, PubMed:25205354, PubMed:27458239). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:1396673, PubMed:15111114). {UniProtKB:P08100, PubMed:1396673, PubMed:16586416, PubMed:16908857, PubMed:17060607, PubMed:17449675, PubMed:18818650, PubMed:21389983, PubMed:22198838, PubMed:23579341, PubMed:25205354, PubMed:27458239, PubMed:10926528, PubMed:11972040, PubMed:12044163, PubMed:15111114, PubMed:26526852}.

Additionnal Information

Immunoblotting: use at 1-10ug/mL. A band of ~40kDa is detected. Immunohistochemistry: use at 1-10ug/mL, paraffin-embedded and frozen. Immunoprecipitation: use at 1-2ug per 100-500ug of protein. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat eye lysate

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes the N-terminus of Rhodopsin from all mammalian species tested (human, mouse, rat, bovine, porcine), and most fish, birds, and amphibians.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Rhodopsin is comprised of the protein opsin and a reversibly covalently bound cofactor, retinal. Opsin, made up of seven membrane-embedded alpha helices, binds retinal, a photoreactive chromophore, in a central pocket. In addition to being the pigment of the retina that is responsible for the formation of photoreceptor cells, rhodopsin conveys information stored in the chromophore to the surface of the molecule upon light absorption. Mutations in the rhodopsin gene lead to retinitis pigmentosa.