Recombinant Human ELN Protein (Trx Tag)

Product Specifications

Background

This gene encodes a protein that is one of the two components of elastic fibers. The encoded protein is rich in hydrophobic amino acids such as glycine and proline, which form mobile hydrophobic regions bounded by crosslinks between lysine residues. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa. Multiple transcript variants encoding different isoforms have been found for this gene.

Abbreviation

ELN

Synonyms

ADCL; ELN; SVAS; WBS; WS; ADCL1; elastin; Tropoelastin; Eps; Elastin peptides; elastin-derived peptides

UniProt

P15502

Accession Number

P15502

Expression System

E.coli

Tag

N-Trx

Sequence

Gly501-Gly551

Field of Research

Signal Transduction; Cardiovascular

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.