Human Laminin 221 Protein, premium grade

Laminin-221 (LM221) takes multiple roles as a scaffold to store cells, growth factors and cytokines and is the most likely expressed cardiac laminin that maintains physiological activities of myocytes. Laminin 221-E8 consists of the C-terminal end of the alpha 2, beta 2, and gamma 1 chains. Previous study has confirmed that the laminin-221 heterotrimer (α2β2γ1) could bind integrin α7X1 and α7X2. Latest research indicated that the administration of LM221 enhanced accumulation of smooth muscle actin (SMA) and isolectin B4 (ILB4) double-positive cells to the damaged area and contributed to the improvement of cardiac function.

Product Specifications

Background

Specifications

Human Laminin 221 Protein, premium grade, produced by co-expression of Laminin alpha2E8, Laminin beta2E8 and Laminin gamma1E8, has a calculated MW of 94.3 kDa, 51.3 kDa and 53.7 kDa. The non-reducing (NR) protein migrates as 118-133 kDa due to glycosylation.

Host

HEK293

Target

Laminin 221

Conjugation

Unconjugated

Source

Human

Stability

-20°C to -70°C for 24 months in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution; 2-8°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.01 EU per μg

Purity

90%

Bioactivity

Immobilized Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) at 5 μg/mL (100 μL/well) can bind Human Laminin 221 Protein, premium grade (Cat. No. LA2-H5269) with a linear range of 0.2-20 ng/mL (QC tested).