Anti-Factor VIII Mouse Monoclonal Antibody - Library Pack

Selection of Mouse Monoclonal antibodies specific to Factor VIII

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Factor VIII Mouse Monoclonal Antibody Library Pack - Includes 100 ug of each of the following antibodies: 10101 - Anti-Factor VIII Mouse Monoclonal Antibody - Clone F82.2.9 - Isotype IgG1 10102 - Anti-Factor VIII Mouse Monoclonal Antibody - Clone F82.2.32 - Isotype IgG1 10103 - Anti-Factor VIII Mouse Monoclonal Antibody - Clone F85.5.72 - Isotype IgG1 10104 - Anti-Factor VIII Mouse Monoclonal Antibody - Clone F827.4 - Isotype IgG2a

Gene Name

Gene ID

300841

NCBI Gene ID

UniProt

P00451

Cellular Locus

Secreted, extracellular space.

Host

Mouse

Reactivity

Human

Immunogen

Purified human Factor VIII.

Target Antigen

Coagulation factor VIII

Target

Factor VIII

Clonality

Monoclonal

Type

Antibody Library Pack

Applications

WB, ELISA

Field of Research

Coagulation factors.

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.

Additionnal Information

These antibodies have been qualified for use in ELISA and immunoblotting to detect human Factor VIII.

Storage Conditions

These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Specificity

10101, 10102, and 10103 recognize full-length human Factor VIII (epitopes not determined). 10104 recognizes an epitope at the N-terminal region of the 83kDa light chain of Factor VIII. These antibodies do not cross-react with von Willebrand factor.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Factor VIII (FVIII) is an essential blood-clotting protein, also known as anti-hemophilic factor (AHF). In humans, factor VIII is encoded by the F8 gene. Defects in this gene result in hemophilia A, a recessive X-linked coagulation disorder. Factor VIII is produced in liver sinusoidal cells and endothelial cells outside of the liver throughout the body. This protein circulates in the bloodstream in an inactive form, bound to another molecule called von Willebrand factor, until an injury that damages blood vessels occurs. In response to injury, factor VIII is activated and separates from von Willebrand factor. The active protein (sometimes written as factor VIIIa) interacts with another coagulation factor called factor IX. This interaction sets off a chain of additional chemical reactions that form a blood clot.