Anti-CIKS/Act1 (NT) Antibody

Rabbit Polyclonal Antibody specific to CIKS/Act1 (NT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 2.3.2.27, Adapter protein CIKS, Connection to IKK and SAPK/JNK, E3 ubiquitin-protein ligase CIKS, Nuclear factor NF-kappa-B activator 1, ACT1, TRAF3-interacting protein 2

Gene Name

TRAF3IP2

NCBI Gene ID

UniProt

O43734

Host

Rabbit

Reactivity

Human

Immunogen

Peptide corresponding to aa 2-15 of human CIKS.

Target Antigen

E3 ubiquitin ligase TRAF3IP2

Target

CIKS/Act1 (NT)

Clonality

Polyclonal

Type

Antibody

Applications

ELISA, WB, IHC-P, IF

Field of Research

Neuroscience

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

E3 ubiquitin ligase that catalyzes 'Lys-63'-linked polyubiquitination of target protein, enhancing protein-protein interaction and cell signaling (PubMed:19825828). Transfers ubiquitin from E2 ubiquitin-conjugating enzyme UBE2V1-UBE2N to substrate protein (PubMed:19825828). Essential adapter molecule in IL17A-mediated signaling (PubMed:19825828, PubMed:24120361). Upon IL17A stimulation, interacts with IL17RA and IL17RC receptor chains through SEFIR domains and catalyzes 'Lys-63'-linked polyubiquitination of TRAF6, leading to TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways (PubMed:19825828). {PubMed:19825828, PubMed:24120361, PubMed:33723527}.

Additionnal Information

Immunoblotting: use at 0.5-1 ug/mL. In immunoblots, a band of 63 kD is detected. <br><br>Positive control: Human placenta tissue lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes an epitope at the N-terminus of human CIKS/Act1 (Connection to IKK and SAPK/JNK), an activator of IKK. CIKS/Act1 interacts directly with IKKg and also activates activating transcription factor (ATF) and activator protein 1 (AP-1) through Jun kinase (JNK). CIKS/Act1 is associated with inflammation and stress responses and is expressed in a variety of human tissues.