BCECF-AM

BCECF-AM is a cell membrane permeable compound widely used as a fluorescent indicator for intracellular pH. BCECF-AM could diffuse through the cell membrane and intracellular esterase cleave the ester bond releasing BCECF (HY-101882) . BCECF allows measurements in the physiological pH range 6.0-8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm.

Product Specifications

CAS Number

[117464-70-7]

UNSPSC

12171500

Hazard Statement

H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/BCECF-AM.html

Concentration

5 mM * 200 μL in DMSO

Purity

99.0

Solubility

10 mM in DMSO

Smiles

O=C(OCOC(C)=O)CCC1=CC(C23OC(C4=C2C=CC(C(OCOC(C)=O)=O)=C4)=O)=C(OC5=C3C=C(CCC(OCOC(C)=O)=O)C(OC(C)=O)=C5)C=C1OC(C)=O.O=C(OCOC(C)=O)CCC6=CC(C78OC(C9=C7C=C(C(OCOC(C)=O)=O)C=C9)=O)=C(OC%10=C8C=C(CCC(OCOC(C)=O)=O)C(OC(C)=O)=C%10)C=C6OC(C)=O

Molecular Formula

C40H36O19

Molecular Weight

820.70

Precautions

H315, H319, H335

References & Citations

[1]Clark Undem, et al. Endothelin-1 Augments Na+/H+ Exchange Activity in Murine Pulmonary Arterial Smooth Muscle Cells via Rho Kinase. PLoS One. 2012; 7 (9) : e46303.|[2]Scott DA, et al. Analysis of the uptake of the fluorescent marker 2',7'-bis- (2-carboxyethyl) -5 (and-6) -carboxyfluorescein (BCECF) by hydrogenosomes in Trichomonas vaginalis. Eur J Cell Biol. 1998 Jun;76 (2) :139-45.|[3]Chen F, et al. CYP51A1 drives resistance to pH-dependent cell death in pancreatic cancer. Nat Commun. 2025 Mar 7;16 (1) :2278. |[4]Pang X, et al. Weak acids produced during anaerobic respiration suppress both photosynthesis and aerobic respiration. Nat Commun. 2023 Jul 14;14 (1) :4207.