Anti-UVRAG Antibody

Versatile protein that is involved in regulation of different cellular pathways implicated in membrane trafficking. Involved in regulation of the COPI-dependent retrograde transport from Golgi and the endoplasmic reticulum by associating with the NRZ complex; the function is dependent on its binding to phosphatidylinositol 3-phosphate (PtdIns(3)P) (PubMed:16799551, PubMed:18552835, PubMed:20643123, PubMed:24056303, PubMed:28306502). During autophagy acts as regulatory subunit of the alternative PI3K complex II (PI3KC3-C2) that mediates formation of phosphatidylinositol 3-phosphate and is believed to be involved in maturation of autophagosomes and endocytosis. Activates lipid kinase activity of PIK3C3 (PubMed:16799551, PubMed:20643123, PubMed:24056303, PubMed:28306502). Involved in the regulation of degradative endocytic trafficking and cytokinesis, and in regulation of ATG9A transport from the Golgi to the autophagosome; the functions seems to implicate its association with PI3KC3-C2 (PubMed:16799551, PubMed:20643123, PubMed:24056303). Involved in maturation of autophagosomes and degradative endocytic trafficking independently of BECN1 but depending on its association with a class C Vps complex (possibly the HOPS complex); the association is also proposed to promote autophagosome recruitment and activation of Rab7 and endosome-endosome fusion events (PubMed:18552835, PubMed:28306502). Enhances class C Vps complex (possibly HOPS complex) association with a SNARE complex and promotes fusogenic SNARE complex formation during late endocytic membrane fusion (PubMed:24550300). In case of negative-strand RNA virus infection is required for efficient virus entry, promotes endocytic transport of virions and is implicated in a VAMP8-specific fusogenic SNARE complex assembly (PubMed:24550300). {PubMed:18552835, PubMed:20643123, PubMed:24056303, PubMed:28306502, PubMed:22542840}.

Immunoblotting: use at 1- 2ug/mL. A band of ~75- 90kDa is detected. <br><br>Immunofluorescence: use at 10ug/mL. <br><br>These are recommended concentrations. <br><br>Endusers should determine optimal concentrations for their applications.

Autophagy is a catabolic process that results in the degradation of bulk cytoplasmic contents within autophagosomes and lysosomes. UV radiation resistance-associated gene product (UVRAG) is a protein localized in the endoplasmic reticulum (ER) and endosomes and is known to regulate autophagosome maturation as well as early stages of autophagy. UVRAG regulates autophagosome maturation by binding to the homotypic fusion and vacuole protein sorting (HOPS) complex, which consists of the class C Vps complex (Vps11-Vps16-Vps18-Vps33) and two additional proteins, Vps39 and Vps41. UVRAG binding to the HOPS complex stimulates lysosomal fusion with autophagosome and endosome.