Human LIF Protein, Tag Free
Human LIF Protein, Tag Free (LIF-H521b) is expressed from human 293 cells (HEK293) . It contains AA Ser 23 - Phe 202 (Accession # AAH69540.1) .
Product Specifications
Background
Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.
Specifications
This protein carries no "tag". The protein has a calculated MW of 19.9 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
AAH69540.1
Expression Region
Ser 23 - Phe 202
Host
HEK293
Target
LIF
Conjugation
Unconjugated
Tag
Native
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
0.1 EU per μg
Purity
90%
Bioactivity
Immobilized Human LIF Protein, Tag Free (Cat. No. LIF-H521b) at 0.2 μg/mL (100 μL/well) can bind Human LIF R, Fc Tag (Cat. No. LIR-H4252) with a linear range of 4-500 ng/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
19.9 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
20ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/LIF-H521b.pdf
Species
Human
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
