1,5-IAEDANS (Highly Pure)

<strong>1,5-IAEDANS (Highly Pure)</strong>_x000D_ <strong>Catalog number:</strong> B2016143_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 25 mg_x000D_ <strong>Molecular Weight or Concentration:</strong> 434.25 g/mol_x000D_ <strong>Supplied as:</strong> Powder_x000D_ <strong>Applications:</strong> a molecular tool for various biochemical applications_x000D_ <strong>Storage:</strong> −20°C_x000D_ <strong>Keywords:</strong> N-(Iodoacetaminoethyl)-1-naphthylamine-5-sulfonic acid, 5-((((2-Iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid, 1-Naphthalenesulfonic acid, 5-((2-((iodoacetyl)amino)ethyl)amino_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Lui SM, Cowan JA. Conformational gating of the dissimilatory sulfite reductase from Desulfovibrio vulgaris (Hildenborough). Synthesis, characterization, and stopped-flow kinetics studies of 1,5-IAEDANS-labeled desulfoviridin Biochemistry. 1994 Sep 20;33(37):11209-16._x000D_ 2: Ajtai K, Burghardt TP. Probe studies of the MgADP state of muscle cross-bridges: microscopic and wavelength-dependent fluorescence polarization from 1,5-IAEDANS-labeled myosin subfragment 1 decorating muscle fibers Biochemistry. 1987 Jul 14;26(14):4517-23._x000D_ 3: Borovikov YuS, Wrotek M, Aksenova NB, Lebedeva NN, Kakol I. Influence of Mg2+ and Ca2+ bound to 1,5-IAEDANS-labeled phosphorylated and dephosphorylated heavy meromyosin complexed with F-actin on polarized fluorescence of the fluorophore FEBS Lett. 1987 Nov 2;223(2):409-12._x000D_ 4: Takashi R, Duke J, Ue K, Morales MF. Defining the "fast-reacting" thiols of myosin by reaction with 1, 5 IAEDANS Arch Biochem Biophys. 1976 Jul;175(1):279-83._x000D_ 5: Tao T. Nanosecond fluorescence depolarization studies on actin labeled with 1,5-IAEDANS and dansyl chloride. Evidence for label flexibility FEBS Lett. 1978 Sep 1;93(1):146-50._x000D_ 6: Borovikov IuS, Novak E, Khoroshev MI, Dabrowska R. [Caldesmon and myosin subfragment-1 act differently on the structural state of 1,5-IAEDANS-modified tropomyosin in ghost muscle fibers] Biokhimiia. 1990 Jul;55(7):1294-8._x000D_ 7: Balestrieri C, Giovane A, Quagliuolo L, Servillo L. Fluorescent labeling of tRNA by 5-[2-(2-iodoacetamido) ethylamino]-1-naphthalenesulfonate (IAEDANS) Ital J Biochem. 1990 May-Jun;39(3):177A-178A._x000D_ 8: Kehoe JJ, Brodkorb A, Mollé D, Yokoyama E, Famelart MH, Bouhallab S, Morris ER, Croguennec T. Determination of exposed sulfhydryl groups in heated beta-lactoglobulin A using IAEDANS and mass spectrometry J Agric Food Chem. 2007 Aug 22;55(17):7107-13._x000D_ 9: Yoshimura H, Mihashi K. The binding of myosin subfragment-1 to F-actin in the absence of nucleotide. Evidence for dependence on F-actin concentration J Biochem. 1982 Aug;92(2):497-508._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/29510086">10: Simonyan AO, Sirenko VV, Karpicheva OE, Robaszkiewicz K, Śliwinska M, Moraczewska J, Krutetskaya ZI, Borovikov YS. The primary cause of muscle disfunction associated with substitutions E240K and R244G in tropomyosin is aberrant behavior of tropomyosin and response of actin and myosin during ATPase cycle Arch Biochem Biophys. 2018 Apr 15;644:17-28. </a>_x000D_ _x000D_ <strong>Products Related to 1,5-IAEDANS (Highly Pure) can be found at</strong> <a href="https://moleculardepot.com/product-category/Fluorescence/"> Fluorescence</a>