Mouse anti Human CDC25A
Product Specifications
Background
The immunogen for the anti CDC25A was a purified recombinant CDC25A protein recognizing a 65kD protein. In the cell cycle of eukaryotes the M phase is initiated by maturation promoting factor (MPF), a protein kinase complex consisting of p34 cdc2, cyclin B, and p13. The activity of this complex is controlled by multiple levels of phosphorylation and dephosphorylation of the catalytic p34cdc2 component. CDC25A and CDC25B arep34cdc2 tyrosine phosphates. Dephosphorylation of p34cdc2 by these CDC25 proteins activates the MPF complex, initiating mitosis. Although expressed throughout the cell cycle, peak expression of CDC25A is in G1 of the cell cycle.
Synonyms
Dual specificity phosphatase Cdc25A, M-phase inducer phosphatase 1, MPIP1,
UniProt
P30304 (Human)
Host
Mouse
Species Reactivity
Human
Isotype
IgG2a
Clone
DCS121
Conjugation
Unconjugated
Type
Monoclonal Antibody
Source
Mice were immunized with a purified recombinant CDC25A protein of 65kD protein
Applications
WB, IP
Field of Research
Cell Cycle
Purification Method
Protein A/G Chromatography
Assay Principle
Western Blot, Immunoprecipitation at 1-10 µg/mL
Stability
See expiration date on vial
Concentration
See vial for Concentration
Form
Provided as solution in phosphate buffered saline with 0,08% sodium azide
Precautions
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
References & Citations
1. Wang, Z., et al. 'Overexpression of CDC25B, CDC25C and phospho-CDC25C (Ser216) in vulvar squamous cell carcinomas are associated with malignant features and aggressive cancer phenotypes.' BMC Cancer 2010, 10, 233_x000D_
2. Berthet, C., et al. 'How adeno-associated virus Rep78 protein arrests cells completely in S phase.' Proc. Natl. Acad. Sci. 2005, 102, 13634-13639
Shipping Conditions
Ambient Temperature
Storage Conditions
Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
Functional Analysis
WB
CAS Number
9007-83-4
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
MBS6266200-01 0.1 mL
CD35 (CR1, Follicular Dendritic Cell Marker) (BSA & Azide Free) (Biotin)
Ask
View Details