Mouse anti Human CDC25A

Product Specifications

Background

The immunogen for the anti CDC25A was a purified recombinant CDC25A protein recognizing a 65kD protein. In the cell cycle of eukaryotes the M phase is initiated by maturation promoting factor (MPF), a protein kinase complex consisting of p34 cdc2, cyclin B, and p13. The activity of this complex is controlled by multiple levels of phosphorylation and dephosphorylation of the catalytic p34cdc2 component. CDC25A and CDC25B arep34cdc2 tyrosine phosphates. Dephosphorylation of p34cdc2 by these CDC25 proteins activates the MPF complex, initiating mitosis. Although expressed throughout the cell cycle, peak expression of CDC25A is in G1 of the cell cycle.

CAS Number

9007-83-4

Synonyms

Dual specificity phosphatase Cdc25A, M-phase inducer phosphatase 1, MPIP1,

UniProt

P30304 (Human)

Host

Mouse

Species Reactivity

Human

Isotype

IgG2a

Clone

DCS121

Conjugation

Unconjugated

Type

Monoclonal Antibody

Source

Mice were immunized with a purified recombinant CDC25A protein of 65kD protein

Applications

WB, IP

Field of Research

Cell Cycle

Purification Method

Protein A/G Chromatography

Assay Principle

Western Blot, Immunoprecipitation at 1-10 µg/mL

Stability

See expiration date on vial

Concentration

See vial for Concentration

Form

Provided as solution in phosphate buffered saline with 0,08% sodium azide

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

References & Citations

1. Wang, Z., et al. 'Overexpression of CDC25B, CDC25C and phospho-CDC25C (Ser216) in vulvar squamous cell carcinomas are associated with malignant features and aggressive cancer phenotypes.' BMC Cancer 2010, 10, 233_x000D_ 2. Berthet, C., et al. 'How adeno-associated virus Rep78 protein arrests cells completely in S phase.' Proc. Natl. Acad. Sci. 2005, 102, 13634-13639