RNA Sample Loading Buffer (Molecular Biology Grade)

<strong>RNA Sample Loading Buffer </strong>_x000D_ <strong>Catalog number:</strong> B2010118_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Volume/Weight:</strong> 4 x 1 mL_x000D_ <strong>pH:</strong> n/a_x000D_ <strong>Supplied as:</strong> Concentrate (Dilute 1:3 or 1:6 with sample before use)_x000D_ <strong>Appearance:</strong> Blue Solution_x000D_ <strong>Applications:</strong> RNA loading buffer during RNA electrophoresis._x000D_ <strong>Storage:</strong> −20°C_x000D_ <strong>Keywords:</strong> RNA loading buffer, RNA electrophoresis buffer_x000D_ <strong>Grade:</strong> Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered on a 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Studier FW, Moffatt BA. Use of bacteriophage T7 RNA polymerase to direct_x000D_ selective high-level expression of cloned genes. J Mol Biol. 1986 May_x000D_ 5;189(1):113-30._x000D_ _x000D_ 2: Borkotoky S, Murali A. The highly efficient T7 RNA polymerase: A wonder_x000D_ macromolecule in biological realm. Int J Biol Macromol. 2018 Oct 15;118(Pt_x000D_ A):49-56._x000D_ _x000D_ 3: Gholamalipour Y, Karunanayake Mudiyanselage A, Martin CT. 3′ end additions by_x000D_ T7 RNA polymerase are RNA self-templated, distributive and diverse in character-_x000D_ RNA-Seq analyses. Nucleic Acids Res. 2018 Oct 12;46(18):9253-9263._x000D_ _x000D_ 4: Sarcar SN, Miller DL. A specific, promoter-independent activity of T7 RNA_x000D_ polymerase suggests a general model for DNA/RNA editing in single subunit RNA_x000D_ Polymerases. Sci Rep. 2018 Sep 17;8(1):13885._x000D_ _x000D_ 5: Liang X, Li C, Wang W, Li Q. Integrating T7 RNA Polymerase and Its Cognate_x000D_ Transcriptional Units for a Host-Independent and Stable Expression System in_x000D_ Single Plasmid. ACS Synth Biol. 2018 May 18;7(5):1424-1435._x000D_ _x000D_ 6: Milisavljevič N, Perlíková P, Pohl R, Hocek M. Enzymatic synthesis of base-_x000D_ modified RNA by T7 RNA polymerase. A systematic study and comparison of_x000D_ 5-substituted pyrimidine and 7-substituted 7-deazapurine nucleoside_x000D_ triphosphates as substrates. Org Biomol Chem. 2018 Aug 15;16(32):5800-5807._x000D_ _x000D_ 7: Jin H, Lindblad P, Bhaya D. Building an Inducible T7 RNA Polymerase/T7_x000D_ Promoter Circuit in Synechocystis sp. PCC6803. ACS Synth Biol. 2019 Apr_x000D_ 19;8(4):655-660._x000D_ _x000D_ 8: Ji S, Thomforde J, Rogers C, Fu I, Broyde S, Tretyakova NY. Transcriptional_x000D_ Bypass of DNA-Protein and DNA-Peptide Conjugates by T7 RNA Polymerase. ACS Chem_x000D_ Biol. 2019 Dec 20;14(12):2564-2575._x000D_ _x000D_ 9: Juhas M, Ajioka JW. T7 RNA polymerase-driven inducible cell lysis for DNA_x000D_ transfer from Escherichia coli to Bacillus subtilis. Microb Biotechnol. 2017_x000D_ Nov;10(6):1797-1808._x000D_ _x000D_ 10: Hassan MI, McSorley FR, Hotta K, Boddy CN. Inducible T7 RNA Polymerase-_x000D_ mediated Multigene Expression System, pMGX. <a href="https://pubmed.ncbi.nlm.nih.gov/28715370/">J Vis Exp.</a> 2017 Jun 27;(124):55187._x000D_ protein synthesis reveals insights into the regulation of T7 RNA p_x000D_ _x000D_ <strong>Products Related to RNA Sample Loading Buffer </strong> <a>Biochemical Buffers and Solutions</a>