Mouse CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (MALS verified)

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Product Specifications

Background

Specifications

Mouse CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 15.1 kDa (CD3E) and 14.3 kDa (CD3D) . Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D is fused with a flag tag at the C-terminus. As a result of glycosylation, the heterodimer protein migrates as 18-19 kDa and 25-32 kDa under reducing (R) condition, and 38-47 kDa under non-reducing (NR) condition (SDS-PAGE) .

Accession Number

P22646-1 (CD3E) & P04235-1 (CD3D)

Expression Region

Asp 23 - Asp 108 (CD3E) & Phe 22 - Ala 105 (CD3D)

Host

HEK293

Target

CD3E & CD3D

Conjugation

Unconjugated

Tag

C-10xHis | C-Flag

Source

Mouse

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Mouse CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (Cat. No. CDD-M5219) at 2 μg/mL (100 μL/well) can bind CD3E Monoclonal Antibody (145-2C11), Biotin with a linear range of 0.2-13 ng/mL (QC tested).