Recombinant Mouse Lactase-like protein (Lctl), partial

Product Specifications

Product Name Alternative

(Klotho/lactase-phlorizin hydrolase-related protein)

Abbreviation

Recombinant Mouse Lctl protein, partial

Gene Name

Lctl

UniProt

Q8K1F9

Expression Region

21-540aa

Organism

Mus musculus (Mouse)

Target Sequence

AWRGPPEEASFYYGTFPPGFSWGVGSSAYQTEGAWDEDGKGPSIWDAFTHGRKEQVLGGDTADTACDSYYKVQEDIALLKELQVSHYRFSLSWPRLLPTGVRAEQVNKRGIKFYSDFIDALLKSNITPVVTLHHWDLPQMLQVAYGGWQNVSMTRYFSDYADLCFEVFGDRVKHWLTFSDPRTMVEKGYETGLHAPGLRLQGTGLYVAAHHIIKAHAQAWHSYNNTWRSKQHGLVGISLNCDWGEPVDIDNPDDIEAAERYLQFCLGWFANPIYAGDYPQVMKDHIGTKSAEQGLEMSRLPTFSLQEKSYLKGTSDFLGLGHFTTRYITQRKYPSHQGPSYQNDRDLVELVDPNWPEMGSPWLYSVPWGFRRLLNFAQTQYGDPPIYVTESGAPQKLHCTQFCDEWRIQYLKGYINEMLKAIKDGVDIKGYTSWSLLDKFEWEKGYADKYGFYYVEFNVRNKPRYPKASVQYYKEIITASGFPNPQEVESWRLKALETCSINNQMLATEPLLRHMHVASE

Tag

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Others

Relevance

Plays a role in formation of the lens suture in the eye, which is important for normal optical properties of the lens.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

64.8 kDa

References & Citations

"Large-scale gene trapping in C57BL/6N mouse embryonic stem cells." Hansen G.M., Markesich D.C., Burnett M.B., Zhu Q., Dionne K.M., Richter L.J., Finnell R.H., Sands A.T., Zambrowicz B.P., Abuin A. Genome Res 18:1670-1679 (2008)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.