PNGase F
Peptide -N-Glycosidase F, also known as PNGase F, is an amidase that cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins
Product Specifications
Expression System
E. coli
Nature
Recombinant
Applications
Removal of high mannose N-glycans from glycoproteins
Stability
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
Endotoxin
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Bioactivity
One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 μg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10 μl.
Form
Liquid
Buffer
20mM Tris-HCl, pH 7.5, 50mM NaCl, 5mM Na2EDTA, 50% glycerol
Shipping Conditions
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Notes
For research use only.
Species
Elizabethkingia miricola
Frequently Asked Questions
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