Proadifen (hydrochloride)

Proadifen (SKF-525A) hydrochloride is a non-competitive Cytochrome P450 inhibitor with an IC50 value of 19 μM. Proadifen hydrochloride reduces monoamine oxidase A (MAO-A) activity and reverses the antidepressantlike behavioral effect of Imipramine (HY-B1490A) and Desipramine (HY-B1272A) in rats. Proadifen hydrochloride also reduces N, N-dimethyltryptamine (DMT) metabolism in liver microsomes and inhibits N-demethylationand Acridone (HY-W007771) formation. Proadifen hydrochloride augments Lipopolysaccharide (LPS) (HY-D1056) -induced fever and exacerbates Prostaglandin E2 (PGE2) (HY-101952) levels in the rat. Proadifen hydrochloride is promising for research of metabolism-related deseases, ovarian carcinoma, inflammation and dopamine neurons-related deseases[1][2][3][4][5][6].

Product Specifications

CAS Number

[62-68-0]

Product Name Alternative

SKF-525A; U-5446; RP-5171

UNSPSC

12352005

Hazard Statement

H302

Target

Bcl-2 Family; Cytochrome P450; Monoamine Oxidase; PARP; Survivin

Type

Reference compound

Related Pathways

Apoptosis; Cell Cycle/DNA Damage; Epigenetics; Metabolic Enzyme/Protease; Neuronal Signaling

Field of Research

Cancer; Inflammation/Immunology; Neurological Disease

Assay Protocol

https://www.medchemexpress.com/Proadifen-hydrochloride.html

Concentration

10mM

Purity

99.95

Solubility

DMSO : 50 mg/mL (ultrasonic)

Smiles

O=C(OCCN(CC)CC)C(CCC)(C1=CC=CC=C1)C2=CC=CC=C2.[H]Cl

Molecular Formula

C23H32ClNO2

Molecular Weight

389.96

Precautions

H302

References & Citations

[1]Grinchii D, et al. Inhibition of cytochrome P450 by proadifen diminishes the excitability of brain serotonin neurons in rats[J]. Gen Physiol Biophys. 2018 Sep;37 (6) :711-713.|[2]Eckernäs E, et al. N, N-dimethyltryptamine forms oxygenated metabolites via CYP2D6 - an in vitro investigation[J]. Xenobiotica. 2023 Dec;53 (8-9) :515-522. |[3]Robertson IG, et al. Inhibition by SKF-525A of the aldehyde oxidase-mediated metabolism of the experimental antitumour agent acridine carboxamide[J]. Biochem Pharmacol. 1993 May 25;45 (10) :2159-62. |[4]Paliokha R, et al. Inhibition of cytochrome P450 with proadifen alters the excitability of brain catecholamine-secreting neurons[J]. Gen Physiol Biophys. 2022 May;41 (3) :255-262. |[5]Kozak W, et al. Proadifen (SKF-525A), an inhibitor of cytochrome P-450, augments LPS-induced fever and exacerbates prostaglandin-E2 levels in the rat[J]. Journal of Thermal Biology, 2000, 25 (1-2) : 45-50.|[6]Jendželovský R, et al. Proadifen sensitizes resistant ovarian adenocarcinoma cells to cisplatin[J]. Toxicology Letters, 2016, 243: 56-66.