Anti-MADD Antibody

Rabbit Polyclonal Antibody specific to MADD (CT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Differentially expressed in normal and neoplastic cells, Insulinoma glucagonoma clone 20, Rab3 GDP/GTP exchange factor, RabGEF, Rab3 GDP/GTP exchange protein, Rab3GEP

Gene Name

MADD

Gene ID

8567

NCBI Gene ID

UniProt

Q8WXG6

Accession Number

NP_001129415

Cellular Locus

Cell membrane, Cytoplasm, Cell projection, axon

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Peptide corresponding to aa 1570-1588 of human MADD. This peptide sequence is identical to that of DENN and differs by one amino acid with rat GDP/GTP exchange protein RAB3-GEP.

Target Antigen

MAP kinase-activating death domain protein

Target

MADD

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Guanyl-nucleotide exchange factor that regulates small GTPases of the Rab family (PubMed:20937701, PubMed:18559336). Converts GDP-bound inactive form of RAB27A and RAB27B to the GTP-bound active forms (PubMed:20937701, PubMed:18559336). Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms, GTPases involved in synaptic vesicle exocytosis and vesicle secretion (By similarity). Plays a role in synaptic vesicle formation and in vesicle trafficking at the neuromuscular junction (By similarity). Involved in up-regulating a post-docking step of synaptic exocytosis in central synapses (By similarity). Probably by binding to the motor proteins KIF1B and KIF1A, mediates motor-dependent transport of GTP-RAB3A-positive vesicles to the presynaptic nerve terminals (By similarity). Plays a role in TNFA-mediated activation of the MAPK pathway, including ERK1/2 (PubMed:32761064). May link TNFRSF1A with MAP kinase activation (PubMed:9115275). May be involved in the regulation of TNFA-induced apoptosis (PubMed:11577081, PubMed:32761064). {UniProtKB:O08873, UniProtKB:Q80U28, PubMed:11577081, PubMed:18559336, PubMed:20937701, PubMed:32761064, PubMed:9115275}.

Additionnal Information

Immunoblotting : use at 1:250-1:500 dilution. <br><br>Positive control: Whole cell lysate from HeLa or NIH3T3 cells.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human and mouse MADD (200-220 kD).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

MAP kinase-activating death domain protein (MADD) was initially identified as the type 1 tumor necrosis factor receptor (TNFR1). Overexpression of MADD activates MAP kinases ERK and JNK and induces the phosphorylation of cytosolic phospholipase A2. MADD shares 98% homology with DENN (differentially expressed in neoplasic vs. normal cells) which was recently identified as a substrate for c-jun N-terminal kinase 3 (JNK3). MADD has greather than 94% homology with a GDO/GTP exchange protein , Rab3-GEP, and it is 87% homologous with KIAA0358, a brain protein of unknown function. Identification of MADD as a component of the TNFR1 signalling complex and the similarity between MADD and Rab3-GEP provides a connection between TNFR1 activation and downstream MAP kinase activity through a guanine-nucleotide exchange protein.