Ethidium monoazide (bromide)

Ethidium monoazide bromide is a DNA intercalating fluorescent dye that enters bacteria with damaged membranes. Ethidium monoazide bromide can be covalently linked to DNA by photoactivation. Ethidium monoazide bromide stains only dead cells[1]. Ethidium monoazide (bromide) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

Product Specifications

CAS Number

[58880-05-0]

UNSPSC

12171500

Target

DNA Stain

Type

Dye Reagents

Related Pathways

Cell Cycle/DNA Damage

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/ethidium-monoazide-bromide.html

Solubility

10 mM in DMSO

Smiles

CC[N+]1=C(C2=CC=CC=C2)C3=CC(N=[N+]=[N-])=CC=C3C4=C1C=C(N)C=C4.[Br-]

Molecular Formula

C21H18BrN5

Molecular Weight

420.31

References & Citations

[1]Nogva HK, et al. Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5'-nuclease PCR. Biotechniques. 2003 Apr;34 (4) :804-8, 810, 812-3.