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Anti-Hu CD207 Alexa Fluor® 488

CD207 is a 40 kDa type II transmembrane glycoprotein of the C-type lectin family. It binds to mannose-bearing glycoproteins and glycolipids of microbial and viral antigens, including HIV gp120. CD207 is expressed only in Langerhans cells, which are immature dendritic cells of the epidermis and mucosa, and its expression decreases during Langerhans cells maturation. Upon antigen binding CD207 localizes to the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed membranes, which leads to a nonclassical antigen-processing pathway.

Product Specifications

Certification

RUO

Immunogen

Fusion protein of human CD207 extracellular part and IgG Fc fragment

Target Antigen

CD207

Clone

2G3

Applications

FC-IC, ICC, IHC-F, FC (QC tested)

Format

Alexa Fluor® 488

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Epaulard O, Adam L, Poux C, Zurawski G, Salabert N, Rosenbaum P, Dereuddre-Bosquet N, Zurawski S, Flamar AL, Oh S, Romain G, Chapon C, Banchereau J, Lévy Y, Le Grand R, Martinon F: Macrophage- and neutrophil-derived TNF-α instructs skin langerhans cells to prime antiviral immune responses. J Immunol. 2014 Sep 1;193 (5) :2416-26. , URL: https://pubmed.ncbi.nlm.nih.gov/25057007/, *Adam L, Tchitchek N, Todorova B, Rosenbaum P, Joly C, Poux C, Chapon C, Spetz AL, Ustav M, Le Grand R, Martinon F: Innate molecular and cellular signature in the skin preceding long-lasting T cell responses after electroporated DNA vaccination. J Immunol. 2020 Jun 15;204 (12) :3375-3388., URL: https://pubmed.ncbi.nlm.nih.gov/32385135/

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody 2G3 recognizes an extracellular/luminal epitope of CD207, a transmembrane protein selectively expressed by Langerhans cells and by some other dendritic cell subsets. This antibody clone can target vaccine-peptides to dendritic cells.

Applications Notes

6

Species

Human, Non-human primates

CAS Number

9007-83-4

Isotype

Mouse IgG1 lambda

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