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Recombinant Human GLO1

Product Specifications

Product Name Alternative

Aldoketomutase, glo1, GLOD1, Glx I, GLYI, glyoxalase domain containing 1, Glyoxalase I, Ketone aldehyde mutase, Ketone-aldehyde mutase, Lactoyl glutathione lyase

UniProt

Q04760

Expression Region

2-184

Host

E.Coli

Tag

N-terminal His Tag

Applications

Western Blot, ELISA

Field of Research

Cancer, Metabolism

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Measured by its ability to catalyze the formation of S-D-lactoylglutathione from the hemimercaptal adduct that forms spontaneously between methylglyoxal and reduced glutathione.

Form

Lyophilized powder

Reconstitution

Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

Molecular Weight

22.5 kDa

Shipping Conditions

4°C with ice bag

Storage Conditions

The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.

Formulation

Lyophilized from a 0.2 μm filtered solution of 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4.

Organism Species

Human

Available Sizes

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