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Anti-Glutathione-S-Transferase (GST) Antibody, HRP conjugate

Selected Variant

18844P-1

Size: 100 µg

Rabbit Polyclonal Antibody specific to Glutathione-S-Transferase (GST) HRP

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Glutathione-S-Transferase (GST) HRP Rabbit Polyclonal Antibody

UniProt

P26624

Host

Rabbit

Reactivity

Glutathione-S-Transferase (Gst)

Immunogen

Glutathione-S-Transferase (GST) from Schistosoma japonicum.

Target Antigen

Glutathione S-transferase class-mu 28 kDa isozyme

Target

Glutathione-S-Transferase (GST)

Clonality

Polyclonal

Isotype

Whole IgG

Type

Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Epitope Tags

Purification Method

Purified by affinity on GST

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.; GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.

Additionnal Information

Immunoblotting: use at a dilution of 1:1,000- 1:30,000. <br><br>Immunocytochemistry: use at a dilution of 1:200-1:500 <br><br>ELISA: use at a dilution of 1:10,000- 1:100,000 <br><br>These are recommended dilutions. <br><br>Endusers should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at 2-8°C.

Specificity

Antibody was affinity-purified on GST.

Formulation

Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400

Buffer pH

pH 7.2

Target Background

A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids which, compared to tags such as the myc-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. Many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.

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