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AKT (Phospho-Y474) polyclonal antibody

Rabbit polyclonal antibody to AKT (Phospho-Y474)

Product Specifications

Product Name Alternative

RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB; RAC

UniProt

P31749

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human AKT around the phosphorylation site of Tyrosine 474.

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/ml

Dilution

WB: 1:500-1:1000, IHC-P: 1:50-1:200

Form

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2

Molecular Weight

~ 60 kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Tested Applications

IHC, WB

Host or Source

Rabbit

Preservative

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2

Available Sizes

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