Mononucleosomes (H1.2)

<strong>Mononucleosomes (H1.2)</strong>_x000D_ <strong>Catalog number:</strong> B2016721_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 10 µg_x000D_ <strong>Molecular Weight or Concentration:</strong> histone octamer and histone H1.2 are 108 kDa and 21.4 kDa, respectively_x000D_ <strong>Supplied as:</strong> Solution_x000D_ <strong>Applications:</strong> a molecular tool for various biochemical applications_x000D_ <strong>Storage:</strong> -80°C_x000D_ <strong>Keywords:</strong> Recombinant Mononucleosomes (H1.2)_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Bröhm A, Schoch T, Dukatz M, Graf N, Dorscht F, Mantai E, Adam S, Bashtrykov P, Jeltsch A. Methylation of recombinant mononucleosomes by DNMT3A demonstrates efficient linker DNA methylation and a role of H3K36me3 Commun Biol. 2022 Mar 2;5(1):192._x000D_ 2: Ruh MF, Chrivia JC, Cox LK, Ruh TS. The interaction of the estrogen receptor with mononucleosomes Mol Cell Endocrinol. 2004 Feb 12;214(1-2):71-9._x000D_ 3: Burdett H, Foglizzo M, Musgrove LJ, Kumar D, Clifford G, Campbell LJ, Heath GR, Zeqiraj E, Wilson MD. BRCA1-BARD1 combines multiple chromatin recognition modules to bridge nascent nucleosomes Nucleic Acids Res. 2023 Nov 10;51(20):11080-11103._x000D_ 4: Nodelman IM, Patel A, Levendosky RF, Bowman GD. Reconstitution and Purification of Nucleosomes with Recombinant Histones and Purified DNA Curr Protoc Mol Biol. 2020 Dec;133(1):e130._x000D_ 5: Larue RC, Plumb MR, Crowe BL, Shkriabai N, Sharma A, DiFiore J, Malani N, Aiyer SS, Roth MJ, Bushman FD, Foster MP, Kvaratskhelia M. Bimodal high-affinity association of Brd4 with murine leukemia virus integrase and mononucleosomes Nucleic Acids Res. 2014 Apr;42(8):4868-81._x000D_ 6: Pires E, Sharma N, Selemenakis P, Wu B, Huang Y, Alimbetov DS, Zhao W, Wiese C. RAD51AP1 mediates RAD51 activity through nucleosome interaction J Biol Chem. 2021 Jul;297(1):100844._x000D_ 7: Lichota J, Grasser KD. Interaction of maize chromatin-associated HMG proteins with mononucleosomes: role of core and linker histones Biol Chem. 2003 Jul;384(7):1019-27._x000D_ 8: Zandian M, Gonzalez Salguero N, Shannon MD, Purusottam RN, Theint T, Poirier MG, Jaroniec CP. Conformational Dynamics of Histone H3 Tails in Chromatin J Phys Chem Lett. 2021 Jul 8;12(26):6174-6181._x000D_ 9: Ni K, Muegge K. LSH catalyzes ATP-driven exchange of histone variants macroH2A1 and macroH2A2 Nucleic Acids Res. 2021 Aug 20;49(14):8024-8036._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/30585724">10: Osunsade A, Prescott NA, Hebert JM, Ray DM, Jmeian Y, Lorenz IC, David Y. A Robust Method for the Purification and Characterization of Recombinant Human Histone H1 Variants Biochemistry. 2019 Jan 22;58(3):171-176. </a>_x000D_ _x000D_ <strong>Products Related to Mononucleosomes (H1.2) can be found at</strong> <a href="https://moleculardepot.com/product-category/Proteins/"> Proteins</a>