PML (Promyelocytic Leukemia Protein)

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recognizes mouse PML, Mr ~90-106kD, depending on degree of protein modification. Does not crossreact with human. MEF1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with P4296. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.