Lefty A (Left-Right Determination, Factor A (LEFTA)

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recognizes 6X His tagged human LEFTY at 30kD. No reactivity occurs against 6X HIS tag. Significant sequence homology exists between the human and mouse forms of LEFTY. Species sequence homology: mouse and rat.