Collagen Type II (Biotin)

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recognizes human Collagen Type II. Typically less than 1% crossreactivity against other types of collagens was detected by ELISA against puri?ed standards. Some class speci?c anti-collagens may be speci?c for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-?xed, paraf?n embedded tissues. Recognizes most mammalian Type II collagens and has negligible crossreactivity with Type I, III, IV, V and VI collagens. Non-speci?c crossreaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.