ZO-3, Mid (Tight Junction-associated Polypeptide, Zonula Occludens 3) (Biotin)

Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Recognizes human ZO-3 protein with a molecular weight of ~130kD. Crossreactivity with other Zonula Occluden family members has not been observed. Reactivity is confirmed with human T84, HeLa, and canine MDCK cell lysates.