ZO-1 (Mid) (Tight Junction-associated Polypeptide, Zonula Occludens 1) (HRP)

Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Recognizes human ZO-1 protein at ~225kD. Bands of unknown origin may be observed at lower molecular weights in some sample lysates. These bands may represent breakdown products.