Wnt6 (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family, Member 6 (Wnt6) (Biotin)

Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Reacts with the chicken Wnt6 protein. On Western blots, it identifies a band at ~35kD and one at ~45kD. The two bands may represent different isoforms or post-translational modification states. Immunohistochemical and immunofluoresent staining using this antibody is observed in non-neural ectoderm of chick embryos from stages 8 to 14, consistent with the reported expression of Wnt6 mRNA as determined by in situ hybridization. Additional staining is found in ectodermal tissues caudal to the otic level in heart-and gut-forming regions. Reactivity has been confirmed with stage 8-20 chicken embryo homogenates.