Wnt1 (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member) (HRP)

Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Reacts with the human and mouse Wnt1 proteins. On Western blots, it identifies a single band at ~41kD. A band of unknown origin at ~80-90kD is sometimes observed; this may represent a dimerized form of Wnt1. Reactivity has been confirmed with Wnt1-transfected HEK293 cells, GIST882 and SW480 cell lysates, and mouse MS-1 cell lysates.