Wnt1 (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member) (Biotin)

Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Reacts with the human and mouse Wnt1 proteins. On Western blots, it identifies a single band at ~41kD. A band of unknown origin at ~80-90kD is sometimes observed; this may represent a dimerized form of Wnt1. Reactivity has been confirmed with Wnt1-transfected HEK293 cells, GIST882 and SW480 cell lysates, and mouse MS-1 cell lysates.