Ubc9 (Ubiquitin-Conjugating Enzyme 9) (Biotin)

Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Reacts with human, mouse, rat and golden hamster ubiquitin-conjugating enzyme 9 (Ubc9) protein. On Western blots, it identifies the target band at ~18kD. A band at ~13kD is observed in mouse spleen homogenates. Reactivity has been confirmed HEK293, HepG2, HL-60 cell lysates and mouse spleen homogenates.