TLR4 (Toll Like Receptor 4) (HRP)

Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

This antibody, specific for human TLR4, shows a positive result for ELISA and detects 2-3 protein species of ~70 to ~100kD on Western blot analysis using human placenta tissue homogenate, JEG-3 placenta choriocarcinoma, U-937 histocytic lymphoma, BC-1 human B-lymphocyte lymphoma, Nalm-6-pre-B lymphocyte and REH pre-B lymphocyte cell lysates. Reactivity has been confirmed with human placenta tissue homogenate, JEG-3 placenta choriocarcinoma, U-937 histocytic lymphoma, BC-1 human B-lymphocyte lymphoma, Nalm-6-pre-B lymphocyte and REH pre-B lymphocyte cell lysates. This antibody should be theoretically reactive to gorilla, orangutan and chimpanzee TRL4 due to amino acid homology.